Cambial-region-specific expression of the Agrobacterium iaa genes in transgenic aspen visualized by a linked uidA reporter gene.
Identifieur interne : 004830 ( Main/Exploration ); précédent : 004829; suivant : 004831Cambial-region-specific expression of the Agrobacterium iaa genes in transgenic aspen visualized by a linked uidA reporter gene.
Auteurs : H. Tuominen [Suède] ; L. Puech ; S. Regan ; S. Fink ; O. Olsson ; B. SundbergSource :
- Plant physiology [ 0032-0889 ] ; 2000.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Arbres, Glucuronidase, Rhizobium, Végétaux génétiquement modifiés.
- métabolisme : Acides indolacétiques.
- Expression des gènes, Gènes rapporteurs, Phénotype, Technique de Northern, Technique de Southern.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Glucuronidase.
- chemical , metabolism : Indoleacetic Acids.
- genetics : Plants, Genetically Modified, Rhizobium, Trees.
- Blotting, Northern, Blotting, Southern, Gene Expression, Genes, Reporter, Phenotype.
Abstract
The level of indole-3-acetic acid (IAA) was locally modified in cambial tissues of transgenic aspen (Populus tremula L. x Populus tremuloides Michx.). We also demonstrate the use of a linked reporter gene to visualize the expression of the iaa genes. The rate-limiting bacterial IAA-biosynthetic gene iaaM and the reporter gene for beta-glucuronidase (GUS), uidA, were each fused to the cambial-region-specific Agrobacterium rhizogenes rolC promoter and linked on the same T-DNA. In situ hybridization of the iaaM gene confirmed that histochemical analysis of GUS activity could be used to predict iaaM gene expression. Moreover, quantitative fluorometric analysis of GUS activity allowed estimation of the level of de novo production of IAA in transgenic lines carrying a single-copy insert of the iaaM, uidA T-DNA. Microscale analysis of the IAA concentration across the cambial region tissues showed an increase in IAA concentration of about 35% to 40% in the two transgenic lines, but no changes in the radial distribution pattern of IAA compared with wild-type plants. This increase did not result in any changes in the developmental pattern of cambial derivatives or the cambial growth rate, which emphasizes the importance of the radial distribution pattern of IAA in controlling the development of secondary xylem, and suggests that a moderate increase in IAA concentration does not necessarily stimulate growth.
DOI: 10.1104/pp.123.2.531
PubMed: 10859183
PubMed Central: PMC59021
Affiliations:
Links toward previous steps (curation, corpus...)
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Regan</name></author><author><name sortKey="Fink, S" sort="Fink, S" uniqKey="Fink S" first="S" last="Fink">S. Fink</name></author><author><name sortKey="Olsson, O" sort="Olsson, O" uniqKey="Olsson O" first="O" last="Olsson">O. Olsson</name></author><author><name sortKey="Sundberg, B" sort="Sundberg, B" uniqKey="Sundberg B" first="B" last="Sundberg">B. 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Sundberg</name></author></analytic><series><title level="j">Plant physiology</title><idno type="ISSN">0032-0889</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Northern (MeSH)</term><term>Blotting, Southern (MeSH)</term><term>Gene Expression (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Glucuronidase (genetics)</term><term>Indoleacetic Acids (metabolism)</term><term>Phenotype (MeSH)</term><term>Plants, Genetically Modified (genetics)</term><term>Rhizobium (genetics)</term><term>Trees (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides indolacétiques (métabolisme)</term><term>Arbres (génétique)</term><term>Expression des gènes (MeSH)</term><term>Glucuronidase (génétique)</term><term>Gènes rapporteurs (MeSH)</term><term>Phénotype (MeSH)</term><term>Rhizobium (génétique)</term><term>Technique de Northern (MeSH)</term><term>Technique de Southern (MeSH)</term><term>Végétaux génétiquement modifiés (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucuronidase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Indoleacetic Acids</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Rhizobium</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arbres</term><term>Glucuronidase</term><term>Rhizobium</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides indolacétiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Northern</term><term>Blotting, Southern</term><term>Gene Expression</term><term>Genes, Reporter</term><term>Phenotype</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Expression des gènes</term><term>Gènes rapporteurs</term><term>Phénotype</term><term>Technique de Northern</term><term>Technique de Southern</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The level of indole-3-acetic acid (IAA) was locally modified in cambial tissues of transgenic aspen (Populus tremula L. x Populus tremuloides Michx.). We also demonstrate the use of a linked reporter gene to visualize the expression of the iaa genes. The rate-limiting bacterial IAA-biosynthetic gene iaaM and the reporter gene for beta-glucuronidase (GUS), uidA, were each fused to the cambial-region-specific Agrobacterium rhizogenes rolC promoter and linked on the same T-DNA. In situ hybridization of the iaaM gene confirmed that histochemical analysis of GUS activity could be used to predict iaaM gene expression. Moreover, quantitative fluorometric analysis of GUS activity allowed estimation of the level of de novo production of IAA in transgenic lines carrying a single-copy insert of the iaaM, uidA T-DNA. Microscale analysis of the IAA concentration across the cambial region tissues showed an increase in IAA concentration of about 35% to 40% in the two transgenic lines, but no changes in the radial distribution pattern of IAA compared with wild-type plants. This increase did not result in any changes in the developmental pattern of cambial derivatives or the cambial growth rate, which emphasizes the importance of the radial distribution pattern of IAA in controlling the development of secondary xylem, and suggests that a moderate increase in IAA concentration does not necessarily stimulate growth.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10859183</PMID><DateCompleted><Year>2000</Year><Month>09</Month><Day>07</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>123</Volume><Issue>2</Issue><PubDate><Year>2000</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Cambial-region-specific expression of the Agrobacterium iaa genes in transgenic aspen visualized by a linked uidA reporter gene.</ArticleTitle><Pagination><MedlinePgn>531-42</MedlinePgn></Pagination><Abstract><AbstractText>The level of indole-3-acetic acid (IAA) was locally modified in cambial tissues of transgenic aspen (Populus tremula L. x Populus tremuloides Michx.). We also demonstrate the use of a linked reporter gene to visualize the expression of the iaa genes. The rate-limiting bacterial IAA-biosynthetic gene iaaM and the reporter gene for beta-glucuronidase (GUS), uidA, were each fused to the cambial-region-specific Agrobacterium rhizogenes rolC promoter and linked on the same T-DNA. In situ hybridization of the iaaM gene confirmed that histochemical analysis of GUS activity could be used to predict iaaM gene expression. Moreover, quantitative fluorometric analysis of GUS activity allowed estimation of the level of de novo production of IAA in transgenic lines carrying a single-copy insert of the iaaM, uidA T-DNA. Microscale analysis of the IAA concentration across the cambial region tissues showed an increase in IAA concentration of about 35% to 40% in the two transgenic lines, but no changes in the radial distribution pattern of IAA compared with wild-type plants. This increase did not result in any changes in the developmental pattern of cambial derivatives or the cambial growth rate, which emphasizes the importance of the radial distribution pattern of IAA in controlling the development of secondary xylem, and suggests that a moderate increase in IAA concentration does not necessarily stimulate growth.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tuominen</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Puech</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Regan</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Fink</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author 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IdType="pubmed">6326095</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1986 May;81(1):86-91</Citation><ArticleIdList><ArticleId IdType="pubmed">16664813</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1999 Aug;19(3):363-9</Citation><ArticleIdList><ArticleId IdType="pubmed">10476083</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1993 Jan;101(1):313-320</Citation><ArticleIdList><ArticleId IdType="pubmed">12231687</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1991 Jul;17(1):49-60</Citation><ArticleIdList><ArticleId IdType="pubmed">1907871</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 1997 Dec;15(13):1398-401</Citation><ArticleIdList><ArticleId IdType="pubmed">9415894</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1998 Dec;16(6):651-9</Citation><ArticleIdList><ArticleId IdType="pubmed">10069073</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 1998 Apr;1(2):142-8</Citation><ArticleIdList><ArticleId IdType="pubmed">10066569</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1998 May;117(1):113-21</Citation><ArticleIdList><ArticleId IdType="pubmed">9576780</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1996 Jul;31(4):887-95</Citation><ArticleIdList><ArticleId IdType="pubmed">8806418</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1990 Apr;14(4):575-83</Citation><ArticleIdList><ArticleId IdType="pubmed">2102836</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1989 Nov;86(21):8467-71</Citation><ArticleIdList><ArticleId IdType="pubmed">2554318</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1985 Dec 1;4(12):3063-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16453648</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Gen Genet. 1991 Aug;228(1-2):104-12</Citation><ArticleIdList><ArticleId IdType="pubmed">1886604</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1995 Dec;109(4):1179-1189</Citation><ArticleIdList><ArticleId IdType="pubmed">12228661</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1994 Dec;106(4):1511-1520</Citation><ArticleIdList><ArticleId IdType="pubmed">12232425</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1992 Jul;99(3):1062-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16668972</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1987 Dec 20;6(13):3901-7</Citation><ArticleIdList><ArticleId IdType="pubmed">3327686</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Gen Genet. 1990 Jul;222(2-3):329-36</Citation><ArticleIdList><ArticleId IdType="pubmed">1703268</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1993 Jan;21(1):17-26</Citation><ArticleIdList><ArticleId IdType="pubmed">7678759</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1997 Oct;115(2):577-585</Citation><ArticleIdList><ArticleId IdType="pubmed">12223825</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1976 May 7;72:248-54</Citation><ArticleIdList><ArticleId IdType="pubmed">942051</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1990 Aug;93(4):1295-302</Citation><ArticleIdList><ArticleId IdType="pubmed">16667616</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1993 Sep;22(6):1067-85</Citation><ArticleIdList><ArticleId IdType="pubmed">8400126</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9282-6</Citation><ArticleIdList><ArticleId 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Fink</name><name sortKey="Olsson, O" sort="Olsson, O" uniqKey="Olsson O" first="O" last="Olsson">O. Olsson</name><name sortKey="Puech, L" sort="Puech, L" uniqKey="Puech L" first="L" last="Puech">L. Puech</name><name sortKey="Regan, S" sort="Regan, S" uniqKey="Regan S" first="S" last="Regan">S. Regan</name><name sortKey="Sundberg, B" sort="Sundberg, B" uniqKey="Sundberg B" first="B" last="Sundberg">B. Sundberg</name></noCountry><country name="Suède"><noRegion><name sortKey="Tuominen, H" sort="Tuominen, H" uniqKey="Tuominen H" first="H" last="Tuominen">H. Tuominen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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